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Allegro™ Single-Use Mixers



High performance, easy-to-use mixers for manual to fully automated solution preparation


Mixing is a critical operation within biopharmaceutical processes for many applications, ranging from buffer and media preparation, in-process unit operations such as low pH viral inactivation, to final formulation. The Allegro single-use mixers are part of Pall’s expanding range of Allegro products and services providing integrated process solutions throughout the drug production process. The Allegro mixers combine critical requirements for single-use technologies, such as extreme ease-of-use, with established engineering principles and criteria for robust mixer design to deliver the ultimate in mixing performance.


The Allegro mixers are available in 50 L, 200 L, 500 L and 1000 L sizes, in both standard and jacketed stainless steel tote formats, and can incorporate sensors (e.g. pH, conductivity and temperature) for on-line monitoring and control. Sensor signals can be handled locally on the mixer, or alternatively, can be fed directly to an existing Distributed Control System (DCS) or to an Automated Pall MVP single-use system. With the added capability of load cell integration, the Allegro mixers from Pall can be fully integrated to the Allegro MVP automated single-use systems, providing complete automated process monitoring and control solutions for all applications that require mixing (e.g pH adjustment for buffers and virus inactivation).
 

Product Features and Benefits

Feature Benefit
4 pitch blade impeller Efficient, low shear general purpose mixing over a wide range of applications and shear sensitive soluitons
Impeller rotation in clockwise or counter-clockwise direction Flexibility to perform both up-flow and down-flow for floating (low density) and settling (high density) fluid or solids



Feature Benefit
Design principles based on existing Allegro 3D tote format for ease-of-use Very easy and quick to install and remove the single-use systems pre and post-use



Feature Benefit
Inflation of the mixer bag Provides mixing envelope for consistent performance from 50 - 1000 L and the ability to have a gas blanket in operation and mix a range of volumes

Mixer Size 200 L 500 L 1000 L
 
Minimum Volume (L)* 50 100 155
Maximum Volume (L) 200 500 1000
* minimum volume also covers on-line sensors

Feature Benefit
Jacketed Totes Temperature control in appropriate applications



Feature Benefit
Sensor integration Allows in-process monitoring and control through integration with automated Allegro MVP systems



Feature Benefit
Uses existing Allegro film with high clarity and ultra-low extractables Material/validation consistency across all Allegro biocontainer based systems



Feature Benefit
Optional baffles available To assist in reducing air entrapment at high rotation speed for sensitive molecules


The Allegro mixers are designed to provide exceptional mixing performance for a wide range of applications from upstream through the downstream process to formulation and filling. With jacketed and standard totes that can integrate on-line sensor technologies and load cells, potential applications include:
  • Upstream media preparation for cell culture
  • Downstream pH adjustment, virus inactivation and buffer preparation
  • Final formulation mixing
In addition to the mixers, Pall also offers a wide range of complimentary technologies within the Allegro single-use platform (such as filters incorporating state-of-the-art membranes and sterile connection and disconnection devices) providing fully validated, integrated processing solutions from upstream to final formulation and filling. The Allegro mixers can be combined with the Pall MVP system to fully automate mixing and filtration processes, including fluid transfer to and from the mixers. The high performance mixer lends itself especially well to difficult mixing applications (mixing dense powders or high viscosities), applications requiring repeatable, fast mixing performance, or where biologicals sensitive to shear are being mixed.

Application Summary

Type of Mixing Solution 200 L 500 L (150 rpm) 1000 L (150rpm)
Solid – Liquid Allura Red Dye (.05 g/L) 5 seconds (75 rpm) 2 min 5 min
DMEM Media (5.36 g/L) < 7 min (150 rpm) 6 min 11 min
PBS Buffer (9.6 g/L) < 5 min (150 rpm) 4 min 7 min
NaCl (1 M) (58.44 g/L) < 1 min (150 rpm) 7 min 12 min
Liquid – Liquid NaCl (200 g/L - 10 g/L) < 1 min 4 min 6 min